The 38-year-old American, last year's runner-up, was the oldest women's singles entrant in 2018.
The only past Wimbledon champion remaining in the field?
Ninth seed John Isner saved two match points and served 64 aces to overcome Belgian Ruben Bemelmans 6-1 6-4 6-7 (6-8) 6-7 (3-7) 7-5 in three hours 46 minutes to reach the third round. She also won her 18th consecutive match on grass courts dating to 2015 - the second-longest streak of her career (20 from 2002 to 2004).
"It's a wonderful feeling walking out there, I take every match one at a time, I try to put on a good match, try hard every time, and will never underestimate anybody - that keeps me in check".
No. 8 Kevin Anderson beat No. 25 Philipp Kohlschreiber of Germany 6-3, 7-5, 7-5 after losing just eight of 61 points on his first serve, while Gael Monfils of France advanced to the fourth round for the first time by knocking out last year's semifinalist Sam Querrey 5-7, 6-4, 6-4, 6-2. The American fought back from a set and two breaks down only to falter near the end. But Keys won the next five games and broke again to level the set score.
Keys lost nine games on the trot from 5-2 up in the first set to 0-4 down in the second. After the double fault in the first set, Mladenovic appeared to lose her confidence. "I felt my mind go and move on".
The 29-year-old Russian needed treatment after securing the decisive break in the final set, but she was able to block out the pain to seal her upset.
"Of course, I am surprised a little bit", Rodina said afterward, "because I won".
Makarova, who made the quarter-finals four years ago, beat a weary-looking Lucie Safarova of the Czech Republic 4-6, 6-1, 6-4.
The 52nd-ranked Giorgi produced a backhand victor at 4-5, 30-40 in the second set, then won a tiebreaker to force a deciding set.
Isner will face world No.31 Stefanos Tsitsipas, the first Greek man to first Greek man to reach the fourth round of a grand slam in the 50-year professional era, for a quarterfinal berth.
Nick Kyrgios, seeded 15, unleashed 42 winners to see off Robin Haase of the Netherlands 6-3, 6-4, 7-5.