Mass UK rail strikes kick off today across five train companies, as the row over the role of the guard and so-called driver-only operation, continues to spread.
The RMT has raised fears over the safety of passengers if guards are withdrawn from service by the train operators in a bid to save money.
Nick Brown, chief operating officer at GTR, said: "This dispute has been hard for our passengers in particular and we are pleased that we can now move ahead and deliver stability by finally concluding this deal with ASLEF".
'We are pleased with a resolution, which we believe, works for the staff and the company, and we now look forward to working with Southern Rial to restore good industrial relations and deliver the service passengers in the region deserve'. The on-board service concept has been welcomed by our passengers across the board.
Bus and rail passengers will be left feeling the effects of strike action across Wigan today. "The independent rail regulator has said driver-controlled trains, which have been used in this country for more than 30 years, are safe", the spokesman added.
The Rail, Maritime and Transport (RMT) union said today action by members remained "solid" across Southern rail, Greater Anglia, Northern, Merseyrail, and Great Western Railway, which was affected by the walkouts for the first time.
Mick Cash, general secretary of the RMT Union, said: "These strikes are about rail safety and accessibility pure and simple".
Mick Whelan, ASLEF's general secretary, said: "The agreement means we will have a second safety-trained person on every train covered by this agreement except in exceptional circumstances".
A Transport Department spokesperson accused the RMT of attempting to disrupt passengers as part of a "political game".
A survey of over 1,000 businesses for the Rail Delivery Group showed that nearly three out of four fear rail strikes will harm the economy with a third believing they could directly harm their businesses.
The dispute has spread to South Western Railway because the RMT is seeking assurances about working practices when a new fleet of suburban trains are introduced in 2019. "Only this week at the Wimbledon derailment it was the guard on that train who safely evacuated 300 passengers in an emergency situation".